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cytokine treatment fetal human cardiac fibroblasts cfs  (Cell Applications Inc)


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    Cell Applications Inc cytokine treatment fetal human cardiac fibroblasts cfs
    Cytokine Treatment Fetal Human Cardiac Fibroblasts Cfs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 95/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fetal+human+cardiac+fibroblasts+cfs/Human+Cardiac+Fibroblasts%3A+HCF/pm38076279-233-4-11
    Average 95 stars, based on 116 article reviews
    cytokine treatment fetal human cardiac fibroblasts cfs - by Bioz Stars, 2026-10
    95/100 stars

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    In Vitro:

    Article Title: Temporal expression and spatial distribution of the proteoglycan versican during cardiac fibrosis development
    Article Snippet: Images were captured at 63x using an oil objective on LSM 800 Airyscan microscope (Zeiss). .. Fetal human cardiac fibroblasts (CFs) (Cell Applications, CA, #306-05F) and primary human cardiomyocytes (CMs) (PromoCell, Germany, #C-12810) were used for in vitro experiments. .. CFs were cultured in cardiac fibroblast growth medium (Cell Applications, CA, #316-500) containing 1% penicillin and streptomycin (Merck Sigma, Germany, #P0781) at 37°C.



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    cytokine treatment fetal human cardiac fibroblasts cfs  (Cell Applications Inc)
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    Cell Applications Inc cytokine treatment fetal human cardiac fibroblasts cfs
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    Versican ( VCAN ) expression is regulated by cytokines and mechanical stretch in cardiac cells. (A) mRNA expression of versican was upregulated by TGF-β1, and downregulated by TNF-α, and IL-1β in cardiac <t>fibroblasts,</t> and (B) in addition by IL-4 in cardiomyocytes compared to control (Control, n = 3). (C, D) Versican and α-SMA ( ACTA2 ) mRNA expression were increased after biaxial strain of 10% (stretch) for 24 h at 1 Hz frequency in fibroblasts compared to non-stretched cells (Control, n = 9). Gene expression is presented as values relative to Control. Bar graphs represent mean ± SD. One-way ANOVA with Dunnett’s multiple comparisons test (A, B) and Student’s t -test (C, D) were used for statistical analysis. P values < 0.05 were considered statistically significant. *P < 0.05 Control vs. cytokine treatment or stretch.
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    Versican ( VCAN ) expression is regulated by cytokines and mechanical stretch in cardiac cells. (A) mRNA expression of versican was upregulated by TGF-β1, and downregulated by TNF-α, and IL-1β in cardiac <t>fibroblasts,</t> and (B) in addition by IL-4 in cardiomyocytes compared to control (Control, n = 3). (C, D) Versican and α-SMA ( ACTA2 ) mRNA expression were increased after biaxial strain of 10% (stretch) for 24 h at 1 Hz frequency in fibroblasts compared to non-stretched cells (Control, n = 9). Gene expression is presented as values relative to Control. Bar graphs represent mean ± SD. One-way ANOVA with Dunnett’s multiple comparisons test (A, B) and Student’s t -test (C, D) were used for statistical analysis. P values < 0.05 were considered statistically significant. *P < 0.05 Control vs. cytokine treatment or stretch.
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    Versican ( VCAN ) expression is regulated by cytokines and mechanical stretch in cardiac cells. (A) mRNA expression of versican was upregulated by TGF-β1, and downregulated by TNF-α, and IL-1β in cardiac fibroblasts, and (B) in addition by IL-4 in cardiomyocytes compared to control (Control, n = 3). (C, D) Versican and α-SMA ( ACTA2 ) mRNA expression were increased after biaxial strain of 10% (stretch) for 24 h at 1 Hz frequency in fibroblasts compared to non-stretched cells (Control, n = 9). Gene expression is presented as values relative to Control. Bar graphs represent mean ± SD. One-way ANOVA with Dunnett’s multiple comparisons test (A, B) and Student’s t -test (C, D) were used for statistical analysis. P values < 0.05 were considered statistically significant. *P < 0.05 Control vs. cytokine treatment or stretch.

    Journal: Matrix Biology Plus

    Article Title: Temporal expression and spatial distribution of the proteoglycan versican during cardiac fibrosis development

    doi: 10.1016/j.mbplus.2023.100135

    Figure Lengend Snippet: Versican ( VCAN ) expression is regulated by cytokines and mechanical stretch in cardiac cells. (A) mRNA expression of versican was upregulated by TGF-β1, and downregulated by TNF-α, and IL-1β in cardiac fibroblasts, and (B) in addition by IL-4 in cardiomyocytes compared to control (Control, n = 3). (C, D) Versican and α-SMA ( ACTA2 ) mRNA expression were increased after biaxial strain of 10% (stretch) for 24 h at 1 Hz frequency in fibroblasts compared to non-stretched cells (Control, n = 9). Gene expression is presented as values relative to Control. Bar graphs represent mean ± SD. One-way ANOVA with Dunnett’s multiple comparisons test (A, B) and Student’s t -test (C, D) were used for statistical analysis. P values < 0.05 were considered statistically significant. *P < 0.05 Control vs. cytokine treatment or stretch.

    Article Snippet: Fetal human cardiac fibroblasts (CFs) (Cell Applications, CA, #306-05F) and primary human cardiomyocytes (CMs) (PromoCell, Germany, #C-12810) were used for in vitro experiments.

    Techniques: Expressing, Control, Gene Expression